IBI Western Transfer System

WESTERN TRANSFER SYSTEM

*Comes complete with buffer tank, patented vented
lid, western transfer module, filter pads, and
power cords

SPECIFICATIONS

Height Width Length
Unit Dimensions: (IB94500) 13.3cm 15.0cm 16.0cm
Unit Dimensions: (IB95000) 9.0cm 6.5cm 14.0cm
Buffer Capacity: 1200ml
Maximum Gel Size: 10x10cm
Voltage Limit: 100VDC
Current Limit: 500mA

IBI’s Western Transfer Protein System allows rapid transfer of protein or peptide samples or nucleic acids separated by mini-polyacrylamide
gels onto 8x10cm or 10x10cm membranes. The unit accommodates two cassette sandwiches for transfer. Proteins separated
by mini PAGE gels can be transferred within the electrode module by high-intensity electrical fields, while maintaining equal
resolution. Depending on the molecular weight and sequence of the protein/peptide, the transfer should take 60 to 90 minutes. The
unit consists of two hinged, perforated transfer cassettes, each color-coded with 1/8” acrylic and a cassette lock. The cassettes slide
into an electrode module. The distance between the electrodes is 4.5cm. The blotting application is dependent on the voltage applied
over the distance between the electrodes. By keeping the distance between the electrodes at a minimum, higher electrical fields are
produced, thus allowing a more efficient protein transfer. The patented vented lid keeps the buffer cool during transfer; for longer
runs, a stir bar can be added to recirculate the buffer, thereby eliminating heat gradients during the transfer. In extreme cases, our IBI
ice-pack can be inserted into the tank for even better cooling.

ACCESSORIES AND REPLACEMENT PARTS

CATALOG# DESCRIPTION
IB50500 Power Cords, Red and Black
IB94040 Replacement Tank
IB94050 Replacement Gel Capture Device
IB94060 Replacement Vented Lid
IB92072 Replacement Tank Connector Kit
IB92080 Ice Pack
IB95010 Transfer Membrane, Thin Blot Paper - 10 Pack
IB95011 Transfer Membrane, Thick Blot Paper - 10 Pack
IB95020 Transfer Membrane, 0.2μm Nitrocellulose - 10 Pack
IB95021 Transfer Membrane, 0.45μm Nitrocellulose - 10 Pack
IB95030 Transfer Membrane, 0.2μm PVDF - 10 Pack
IB95031 Transfer Membrane, 0.45μm PVDF - 10 Pack
IB95040 Replacement Foam Filter Pads - 4 Pack

MEMBRANE DESCRIPTION

Nitrocellulose
Good binding capacity, proteins bind by hydrophobic interactions
Pore size: .45μm - .22μm
Works well for Western transfers
Amino acid analysis

Nylon
Microporous membrane modified with strongly basic charged groups
Binds negatively charged macromolecules DNA/RNA w/low background
Pore size: .45μm
Can reprobe
Works well for Southern Transfers
Works well for Northern Transfers
Solid-phase immobilization
Enzyme immobilization
Gene probe assays

PVDF
High binding capacity
High hydrophobic binding solvent resistance
Compatible with protein stains and immunodetection techniques
Pore size: .45μm
Can reprobe
Works well for Western Transfers
Protein Sequencing
Amino acid analysis
Solid phase assay systems