IBI AGAROSE, MOLECULAR BIOLOGY GRADE-100GM

IBI Molecular Biology agarose products are highly refined and highly purified. Each lot is physically tested and verified for performance
in its’ designated application. IBI applies strict testing parameters to insure lot to lot consistency. IBI agarose products are
offered in a wide variety of package sizes to fit the needs of any research lab. Each bottle of IBI agarose comes packaged with a
specification sheet, preparation instructions, and MSDS for your convenience. If you require a quantity or specific size of agarose that is not
listed here, just give us a call at 0409077539 or send us an e-mail at sales@labproscientific.com.

PHYSICAL SPECIFICATIONS

CAS#: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9012-36-6
Moisture: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7%
Ash: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0.4%
EEO:. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .05-.13
Sulfate: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10%
Clarity @ 1.5% (NTU): . . . . . . . . . . . . . . . . . . . 3
Gel Strength @ 1.0% (g/cm2): . . . . . . . . . . . . . 1200
Gel Strength @ 1.5% (g/cm2): . . . . . . . . . . . . . 2500
Gelling Temperature @ 1.5%: . . . . . . . . . . . . . 36C ± 1.5C
Melting Temperature @ 1.5%: . . . . . . . . . . . . . 88C ± 1.5C

MOLECULAR BIOLOGY SPECIFICATIONS

DNase assay: . . . . . . . . . . . . . . . . . . . . . . . . . . . None Detected
RNase assay: . . . . . . . . . . . . . . . . . . . . . . . . . . . None Detected
Protease assay:. . . . . . . . . . . . . . . . . . . . . . . . . . None Detected
Gel analysis assay:. . . . . . . . . . . . . . . . . . . . . . . Pass
DNA Resolution: . . . . . . . . . . . . . . . . . . . . . . . . 1000bp (Finely Resolved)
DNA Extraction assay: . . . . . . . . . . . . . . . . . . . Pass
Gel Background: . . . . . . . . . . . . . . . . . . . . . . . . Very Low

STORAGE

Store at room temperature
Protect from moisture
Light Sensitive

FEATURES

Extraordinary mechanical resistance insures safe and easy handling
Pore size can be varied by modifying the gel concentration
Exceptionally low absorption of staining reagents
Excellent transparency allows for high visibility
Greater thermal stability due to high hysteresis

APPLICATIONS

Nucleic Acid, Analytical and Preparative Electrophoresis
Blotting
Protein Electrophoresis, such as Radial Immunodiffusion.

BASE PAIR DATA PREPARATION OF AN AGAROSE GEL AND
ELECTROPHORESIS BUFFER - DNA

1. Select the percentage gel necessary to effectively resolve your sample,
using the following guideline:
GEL CONCENTRATIONS AND RESOLVING RANGES
2. Weigh an appropriate quantity of agarose and place it into a 250ml flask.
NOTE: 0.3% equals 0.3gm of agarose per 100ml of gel volume.
3. Make 500ml of either 1X TAE or 1X TBE electrophoresis buffer.
4. Add ethidium bromide to the diluted electrophoresis buffer to a final
concentration of 0.5μg/ml.
5. Add 6.6ml of the 1X electrophoresis buffer containing ethidium bromide
(made in step 4) per millimeter of gel thickness desired, up to a maximum
of 50ml to a flask containing the agarose.
6. Make note of the total solution volume, so that a degree of evaporation
can be determined and corrected for.
7. Heat the agarose slurry in a microwave oven for approximately 90 seconds.
Swirl the flask to ensure grains sticking to the walls enter into the
solution, heat the solution again for 30-60 seconds if necessary. Swirl
flask again to ensure all the agarose has dissolved into the solution.
8. Add deionized water to replace any volume lost through evaporation during
the heating process. You are ready to pour your gel.

Concentration Efficient Range of
of Agarose in Gel Separations of Linear DNA
(% w/v) (kb)
0.3% 5-60
0.6% 1-20
0.7% .8-10
0.9% .5-7
1.2% .4-6
1.5% .2-3
2.0% .1-2
1X TAE Buffer Gel Concentration 1X TBE Buffer
1500–50bp 2.0 01000–50bp
2500–100bp 1.75 02000–100bp
5000–200bp 1.5 03000–150bp
10,000–250bp 1.25 04000–200bp
16,000–300bp 1.0 08000–300bp
20,000–500bp 0.75 12,000–500bp